Investigation of biological activity of exogenous glutathione on the wheat seed germination by UV-spectroscopy method
Abstract
The biological activity of exogenous glutathione was studied. The growth-stimulating effect of this drug on the initial stages of wheat seed germination was shown. The method of electronic absorption spectroscopy was used to monitor the release of seed products (metabolites) into the cultivation medium (distilled water) at the initial stage of soaking. The spectral characteristics of the resulting metabolites were determined. The rate of metabolite accumulation was recorded in the control and in experiments with different concentrations of glutathione. The study demonstrates a dose-dependent effect of the compound's growth-stimulating action on the rate of metabolite accumulation.
References
Cairns, N. G., Pasternak, M., Wachter, A., Cobbett, C. S., & Meyer, A. J. (2006). Maturation of Arabidopsis seeds is dependent on glutathione biosynthesis within the embryo. Plant physiology, 141(2), 446–455. https://doi.org/10.1104/pp.106.077982.
Szalai, G., Kellős, T., Galiba, G., & Kocsy, G. (2009). Glutathione as an antioxidant and regulatory molecule in plants under abiotic stress conditions. Journal of Plant Growth Regulation, 28(1), 66–80.
Sies, H. (2017). Hydrogen peroxide as a central redox signaling molecule in physiological oxidative stress: Oxidative eustress. Redox biology, 11, 613–619. https://doi.org/10.1016/j.redox.2016.12.035.
Winterbourn C.C., Hampton M.B. (2008). Thiol chemistry and specificity in redox signaling. Free Radical Biol Med. 45, 549. https://doi.org/10.1016/j.freeradbiomed.2008.05.004.
Kasaikina, O. T., Zinatullina, K. M., Kancheva, V. D., Slavova-Kasakova, A. K., & Loshadkin, D. V. (2022). Effect of Lipophilic and Hydrophilic Thiols on the Lipid Oxidation. In Lipid Oxidation in Food and Biological Systems: A Physical Chemistry Perspective (pp. 185–200). Cham: Springer International Publishing.
Shekhter A.B., Rudenko T.G., Serezhenkov V.A., Vanin A.F. (2007). Dinitrosyl-iron complexes with cysteine or glutathione accelerate skin wound healing in animals. Biophysics. 52(3), 539–547.
Martirosyan L.YU., Rubtsova N.A., Smurova L.A., Martirosyan YU.TS., Zinatullina K.M., Lobanov A.V., Kasaikina O.T. (2022). The effect of exogenous glutathione on the regenerative potential of callus tissues of taraxacum kok-saghyz l.e. rodin. Khimicheskaya Bezopasnost’ = Chemical Safety Science, 6(1), 198–207. https://doi.org/10.25514/CHS.2022.1.21014
Aksenov S. I., Golovina E. A. (1996). Penetration and distribution of water in wheat seeds during imbibition. Plant Physiology 33(1), 150–158.
Copyright (c) 2026 Lydia A. Smurova , Viktor V. Vedutenko, and Dmitry А. Krugovov
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
