Экстракт из борщевика Сосновского (Heracleum sosnowskyi Manden.) как стимулятор роста микроорганизмов

Dmitry V.  Gruznov; Olga A. Gruznova; Anton V. Lobanov; Gulizar Sh.  Shcherbakova; Nikolay I.  Popov; Anna F.  Stepnova; Harry Z.  Kaziev; Marina I.  Dinu

doi:10.25514/CHS.2023.2.25001

Dmitry V. Gruznov All-Russian Research Institute for veterinary sanitation, hygiene and ecology – branch of the Federal State Budgetary Scientific Institution Federal Research Center All-Russian Research Institute for Experimental Veterinary Science Russian Academy of Sciences, Moscow, Russia https://orcid.org/0000-0001-6679-9466
Olga A. Gruznova N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences https://orcid.org/0000-0002-0241-1482
Anton V. Lobanov N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, Moscow, Russia; Moscow State Pedagogical University, Moscow, Russia https://orcid.org/0000-0003-4205-7630
Gulizar Sh. Shcherbakova All-Russian Research Institute for veterinary sanitation, hygiene and ecology – branch of the Federal State Budgetary Scientific Institution Federal Research Center All-Russian Research Institute for Experimental Veterinary Science Russian Academy of Sciences, Moscow, Russia https://orcid.org/0000-0003-1324-5341
Nikolay I. Popov All-Russian Research Institute for veterinary sanitation, hygiene and ecology – branch of the Federal State Budgetary Scientific Institution Federal Research Center All-Russian Research Institute for Experimental Veterinary Science Russian Academy of Sciences, Moscow, Russia https://orcid.org/0000-0001-6020-2534
Anna F. Stepnova Moscow Pedagogical State University, Moscow, Russia https://orcid.org/0009-0006-0181-2061
Harry Z. Kaziev Moscow Pedagogical State University, Moscow, Russia https://orcid.org/0009-0007-7952-1819
Marina I. Dinu Moscow Pedagogical State University, Moscow, Russia https://orcid.org/0000-0001-5010-5300

DOI: https://doi.org/10.25514/CHS.2023.2.25001

Keywords: Sosnowsky’s hogweed extract, psoralens, chromatography-mass spectrometry, spectrophotometry, growth stimulator

Abstract

Sosnovsky's hogweed (Heracleum sosnowskyi Manden.) has been considered one of the most common phytoinfestations in Russia for many years. Hogweed isn’t suitable for feeding to farm animals, and also causes contact phytophotodermatitis. Nevertheless, hogweed also has number of positive properties: analgesic, anti-inflammatory and wound healing effects. In addition, the chemical composition of hogweed is quite complex and interesting for research. In connection with the foregoing, as well as the need to solve the problem of curbing the spread and disposal of Sosnovsky's hogweed, it seems relevant to develop methods for obtaining biologically active raw materials from it for subsequent use in various fields. The article presents data on obtaining the aqueous hogweed extract using cavitation, analysis of its composition, as well as the effect on sanitary indicative microorganisms: Escherichia coli (strain 1257), Staphylococcus aureus (strain 209-P) and Bacillus cereus (strain 96). It was found that the aqueous extract is stable during storage at a temperature of 4±2 °C for 9 months. Analysis of the extract by chromatography-mass spectrometry showed the presence of various compounds, most of which were 2-hydroxy-propionic (55.32%) and succinic (23.27%) acids. Using spectrophotometry, 5- and 8-methoxypsoralens were detected in it at 58.1±1.3 µg/ml concentration. The conducting of microbiological test demonstrated the absence of inhibitory effect of the extract on E. coli, S. aureus and B. cereus, including when the pH was changed to 1.5 and 0.5. However, its stimulating effect on these microorganisms was noted, which can be explained by the organic acids content. The results obtained in the study of the effect of extract on E. coli and S. aureus allow us to make the assumption about the possible growth stimulation of similar resistance groups. It can be potential hazard if microorganisms contaminate damaged skin epithelium and mucous membranes. The positive effect of the extract on the soil bacterium B. cereus may also be responsible for the indirect stimulation of agricultural plants growth. However, these assumptions require further research.

References

Madebeykin, I.N. (2021). Hogweed Sosnowski – a dangerous plant that is useful for bees. Pchelovodstvo = Beekeeping, 4, 25 (in Russ).

Tanirbergenov, Т.B., Babushkina, A.E., & Vasyukova, N.S. (2018). Some aspects of pre-clinical studies of Heracleum L. species: review of publications. Voprosy biologicheskoy, meditsinskoy i farmatsevticheskoy khimii = Problems of biological, medical and pharmaceutical chemistry, 21(10), 104–111 (in Russ). https://doi.org/10.29296/25877313-2018-10-20.

Ageev, V.P., Shlyapkina, V.I., Kulikov, O.A., Tararina, L.A., & Zaborovskiy A.V. (2022). Qualitative and quantitative analysis of the main psoralen derivatives in the juice of Sosnowsky's hogweed. Farmatsiya = Pharmacy, 71(3), 10–17 (in Russ). https://doi.org/10/29296/25419218-2022-03-02.

Tamrazova, O.B., Seleznev, S.P., & Tamrazova, A.V. Phytodermatitis in children caused by the Sosnowski hogweed. Pediatriya. Consilium Medicum = Pediatrics. Consilium Medicum, 2, 53–57 (in Russ). https://doi:10.26442/26586630.2019.2.1904182019.

Baker, B.G., Bedford, J. & Kanitkar, S. (2017). Keeping pace with the media; Giant Hogweed burns – A case series and comprehensive review. Journal Burns, 43(5), 933–938. https://doi.org/10.1016/j.burns.2016.10.018.

Kucenko, V.P., Kovaleva, D.D., Mironova, N.R., Ledencova, S.S., Bezvulyak, E.I., & Seliverstov P.V. Sosnovsky's hogweed and photochemical dermatitis. Medicinskaya sestra = Nurse, 24(3), 19–22 (in Russ). https://doi.org/10.29296/25879979-2022-03-03.

Shemyakina, A.V. (2021). Hogweed - honey plant of the Far East. Pchelovodstvo = Beekeeping, 7, 27–28 (in Russ).

Bruni, R., Barreca, D., Protti, M., Brighenti, V., Righetti, L., Anceschi, L., Mercolini, L., Benvenuti, S., Gattuso, G. & Pellati, F. (2019). Botanical sources, chemistry, analysis, and biological activity of furanocoumarins of pharmaceutical interest. Molecules, 24, 2163. https://doi:10.3390/molecules24112163.

Ren, Y., Song, X., Tan, L., Guo, C., Wang, M., Liu, H., Cao, Z., Li, Y. & Peng, C. (2020). A Review of the pharmacological properties of psoralen. Front. Pharmacol., 11, 571535. https://doi.org/10.3389/fphar.2020.571535.

Anosov, A.K., Gonchukov, S.A., Markoliya, A.A., Moshnin, M.V., Parenago, O.O., & Pokrovskiy O.I. (2008). To a problem of PUVA therapy efficiency, Al'manakh klinicheskoy meditsiny = Clinical Medicine Almanac, 17(2), 26–29 (in Russ).

Caffieri, S., Lisa, F., Bolesani, F., Facco, M., Semenzato, G., Dall’Acqua, F. & Canton, M. (2007). The mitochondrial effects of novel apoptogenic molecules generated by psoralen photolysis as a crucial mechanism in PUVA-therapy. Blood, 109(9), 4988–4994. https://doi:10.1182/blood-2006-08-037192.

Rožman, K., Alexander, E.M., Ogorevc, E., Bozovičar, K., Sosič, I., Aldrich, C.C. & Gobec, S. (2020). Psoralen derivatives as inhibitors of mycobacterium tuberculosis proteasome. Molecules, 25(6), 1305. https://doi:10.3390/molecules25061305.

Pliashak, Ye.М., Palianskikh, A.I., Belysheva, L.L., & Baturevich, A.I. (2022).Validation of method for determination of organochlorine pesticides in grain and flour by GC-MS/MS using QuEChERS sample preparation. Khimicheskaya bezopasnost' = Chemical Safety Sciences, 6(2), 243–257 (in Russ). https://doi.org/10.25514/CHS.2022.2.23016.

Bondareva, L.G., Egorchenkova, O.E., & Fedorova, N.E. (2022). Determination of the content of nonionic surfactants in the air by spectrophotometry and gas-liquid chromatography. Khimicheskaya bezopasnost' = Chemical Safety Sciences, 6(1), 148–162 (in Russ). https://doi.org/10.25514/CHS.2022.1.21009.

Gruznova, O.A., Lobanov, A.V., Sokhlikov, A.B., & Gruznov, D.V. (2022). Determination of the correlation between the content of 5-hydroxymethylfurfural and hydrogen peroxide in honey. Khimicheskaya bezopasnost' = Chemical Safety Sciences, 6(2), 215–226 (in Russ). https://doi.org/10.25514/CHS.2022.2.23014.

Bannikova, D. А., Kononenko, А.B., & Lobanov, A.V. (2017). Influence of metallic and metall complex nanoparticles on bacterial populations. Khimicheskaya bezopasnost' = Chemical Safety Sciences, 1(2), 88–96 (in Russ). https://doi.10.25514/CHS.2017.2.10983.

Krupyanskii, Y.F., Generalova, A.A., Kovalenko, V.V. Loiko, N.G., Tereshkin, E.V., Moiseenko, A.V., Tereshkina, K.B., Sokolova, O.S., & Popov, A.N. (2023). DNA condensation in bacteria. Russian Journal of Physical Chemistry B, 42(6), 3-20 (in Russ). https://doi.org/10.31857/S0207401X23060067.

Tertyshnaya, Y.V., Khvatov, A.V., & Lobanov, A.V. (2020). Morphology and Antibacterial Properties of Composites Based on Polylactide and Manganese(III) Complex with Tetraphenylporphyrin. Russian Journal of Physical Chemistry B, 14(6), 1022. https://doi.org/10.1134/S1990793120060135.

Rahnama, H., Azari, R., Yousefi, M.H., Berizi, E., Mazloomi, S.M., Hosseinzadeh, S., Derakhshan, Z., Ferrante M., & Conti G.O. (2023). A systematic review and meta-analysis of the prevalence of Bacillus cereus in foods. Food Control, 143, 109250. https://doi.org/10.1016/j.foodcont.2022.109250.

Vereshchagin, A.L., Kropotkina, V.V., & Khmeleva, A.N. (2010). On the mechanism of growth-stimulating action of ultra-low doses of natural organic acids. Vestnik Altayskogo gosudarstvennogo agrarnogo universiteta = Bulletin of the Altai State Agrarian University, 1(63), 46–48 (in Russ).

Triandafilov, A.F., Chernov, B.A., & Sheshunova, E.V. (2019). Analysis of the composition of lipophilic substances in the juice of hogweed sosnovsky before and after electric discharge cavitation treatment. Vestnik APK Verkhnevolzh'ya = Bulletin of the Agro-Industrial Complex of the Upper Volga Region, 2(46), 66–70 (in Russ). https://doi.org/10.35694/YARCX.2019.46.2.013.

Sosnowsky's hogweed extract (Heracleum sosnowskyi Manden.) as the stimulator of microorganisms growth

Abstract

References